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Test Code SBL Blastomyces Antibody by Immunodiffusion, Serum

Reporting Name

Blastomyces Ab, Immunodiffusion, S

Useful For

Detection of antibodies in patients having blastomycosis

Method Name


Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Forms: If not ordering electronically, complete, print, and send a Neurology Test Request Form (T732) with the specimen (

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To


Mild OK; Gross reject


Mild OK; Gross reject





Reference Values


Day(s) and Time(s) Performed

Monday through Friday; 9:30 a.m.

CPT Code Information


LOINC Code Information

Result ID Reporting Name LOINC Code
15125 Blastomyces Immunodiffusion 5058-3

Clinical Information

The dimorphic fungus, Blastomyces dermatitidis, causes blastomycosis. When the organism is inhaled, it causes pulmonary disease - cough, pain, and hemoptysis, along with fever and night sweats. It commonly spreads to the skin, bone, or internal genitalia where suppuration and granulomas are typical. Occasionally, primary cutaneous lesions after trauma are encountered; however, this type of infection is uncommon.


A positive result is suggestive of infection, but the results cannot distinguish between active disease and prior exposure.


Routine culture of clinical specimens (eg, respiratory specimen) is recommended in cases of suspected, active blastomycosis.


The Mayo experience with the immunodiffusion test is that it is positive in fewer cases of blastomycosis than the 70% to 85% reported elsewhere.


Patients with histoplasmosis may have low-titered cross reactions.

Method Description

Antibodies to the mycelial and yeast forms of Blastomyces dermatitidis are detected in the patient's serum by complement fixation (CF) and immunodiffusion tests.(Kaufman L, Kovacs JA, Reiss E: Clinical immunomycology. In Manual of Clinical and Laboratory Immunology. Edited by NL Rose, E Conway-de Macario, JD Folds, et al. Washington, DC, ASM Press, 1997, pp 587-588; Kaufman L, Mclaughlin DW, Clark MJ, Blumer S: Specific immunodiffusion test for blastomycosis. Appl Microbiol 1973;26:244-247; Williams JE, Murphy R, Standard PG, Phair JP: Serologic response in blastomycosis: diagnostic value of double immunodiffusion assay. Am Rev Resp Dis 1981;123:209-212)

Analytic Time

2 days

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.