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Test Code BMAMA Basic Metabolic Panel, Serum


Necessary Information


Patient's age and sex are required.

Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.


Useful For

Routine health monitoring or patient monitoring while hospitalized for information regarding metabolism, including the current kidney status, electrolyte, and acid/base balance, and blood glucose

Profile Information

Test ID Reporting Name Available Separately Always Performed
KS Potassium, S Yes Yes
NAS Sodium, S Yes Yes
CL Chloride, S Yes Yes
HCO3 Bicarbonate, S Yes Yes
AGAP Anion Gap No Yes
BUN Bld Urea Nitrog(BUN), S Yes Yes
CRTS1 Creatinine with eGFR, S Yes Yes
CA Calcium, Total, S Yes Yes
GLURA Glucose, Random, S Yes Yes

Method Name

KS, NAS, CL: Indirect Potentiometry

BUN, CA, GLURA, HCO3: Photometric

AGAP: Calculation

CRTS1: Colorimetric

Reporting Name

Basic Metabolic Panel, S

Specimen Type

Serum

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated 24 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Clinical Information

The basic metabolic panel measures 8 analytes and calculates an anion gap. It is used to assess kidney status, electrolyte, and acid/base balance, and blood glucose.

Reference Values

SODIUM

<1 year: Not established

≥1 year: 135-145 mmol/L

 

POTASSIUM

<1 year: Not established

≥1 year: 3.6-5.2 mmol/L

 

CHLORIDE

<1 year: Not established

1-17 years: 102-112 mmol/L

≥18 years: 98-107 mmol/L

 

BICARBONATE

Males:

<1 year: Not established

1-2 years: 17-25 mmol/L

3 years: 18-26 mmol/L

4-5 years: 19-27 mmol/L

6-7 years: 20-28 mmol/L

8-17 years: 21-29 mmol/L

≥18 years: 22-29 mmol/L

 

Females:

<1 year: Not established

1-3 years: 18-25 mmol/L

4-5 years: 19-26 mmol/L

6-7 years: 20-27 mmol/L

8-9 years: 21-28 mmol/L

≥10 years: 22-29 mmol/L

 

ANION GAP

<7 years: Not established

≥7 years: 7-15

 

BLOOD UREA NITROGEN

Males:

<12 months: Not established

1-17 years: 7-20 mg/dL

≥18 years: 8-24 mg/dL

 

Females:

<12 months: Not established

1-17 years: 7-20 mg/dL

≥18 years: 6-21 mg/dL

 

CREATININE

Males:

0-11 months: 0.17-0.42 mg/dL

1-5 years: 0.19-0.49 mg/dL

6-10 years: 0.26-0.61 mg/dL

11-14 years: 0.35-0.86 mg/dL

≥15 years: 0.74-1.35 mg/dL

 

Females:

0-11 months: 0.17-0.42 mg/dL

1-5 years: 0.19-0.49 mg/dL

6-10 years: 0.26-0.61 mg/dL

11-15 years: 0.35-0.86 mg/dL

≥16 years: 0.59-1.04 mg/dL

 

ESTIMATED GLOMERULAR FILTRATION RATE (eGFR)

≥ 18 years old: ≥60 mL/min/BSA (body surface area)

Estimated GFR calculated using the 2021 CKD-EPI creatinine equation

Note: eGFR results will not be calculated for patients younger than 18 years old.

 

CALCIUM

<1 year: 8.7-11.0 mg/dL

1-17 years: 9.3-10.6 mg/dL

18-59 years: 8.6-10.0 mg/dL

60-90 years: 8.8-10.2 mg/dL

>90 years: 8.2-9.6 mg/dL

 

GLUCOSE

0-11 months: Not established

≥1 year: 70-140 mg/dL

Interpretation

Basic metabolic panel results are usually evaluated in conjunction with each other for patterns of results. A single abnormal test result could be indicative of something different than if more than 1 of the test results are abnormal. Many conditions will cause abnormal results including kidney failure, breathing problems, and diabetes-related complications.

Cautions

No significant cautionary statements

Method Description

Sodium, Potassium, Chloride:

An ion-selective electrode (ISE) makes use of the unique properties of ion-selective membrane to develop an electrical potential (electromotive force: EMF) for the measurements of ions in solution. Selective membrane is in contact with both the test solution and an internal filling solution. Due to the selectivity of the membrane, only the ions to be measured contribute to the EMF. The membrane EMF is determined by the difference in concentration of the test ion in the test solution and the internal filling solution. The EMF develops according to Nernst equation of a specific ion in solution.(Package insert: ISE indirect Na-K-Cl for Gen 2. Roche Diagnostics; 05/2024)

 

Bicarbonate:

Bicarbonate reacts with phosphoenolpyruvate (PEP) in the presence of PEPC to produce oxaloacetate and phosphate. That reaction is coupled with one involving the transfer of a hydrogen ion from reduced nicotinamide adenine dinucleotide (NADH) analog to oxaloacetate using malate dehydrogenase. The resultant consumption of NADH analog causes a decrease in absorbance, which is proportional to the concentration of bicarbonate in the sample being analyzed.(Package insert: Bicarbonate reagent. Roche Diagnostics; 02/2023)

 

Anion Gap:

This is a calculated result. The following equation is used to calculate the anion gap (A gap):

A gap =Na - (Cl + HCO3[-])

 

Blood Urea Nitrogen:

Kinetic test with urease and glutamate dehydrogenase. Urea is hydrolyzed by urease to form ammonium and carbonate. In the second reaction 2-oxoglutarate reacts with ammonium in the presence of glutamate dehydrogenase and the coenzyme NADH to produce L-glutamate. In this reaction two moles of NADH are oxidized to NAD[+] for each mole of urea hydrolyzed. The rate of decrease in NADH concentrations is directly proportional to the urea concentration in the specimen and is measured photometrically.(Package insert: Urea/BUN reagent. Roche Diagnostics; 07/2024)

 

Creatinine:

This enzymatic method is based on the conversion of creatinine with the aid of creatininase, creatinase, and sarcosine oxidase to form glycine, formaldehyde, and hydrogen peroxide. Catalyzed by peroxidase the liberated hydrogen peroxide reacts with 4-aminophenazone and HTIB (2,4,6-triiodo03-hydroxybenzoic acid) to form a quinone imine chromogen. The color intensity of the quinone imine chromogen formed is directly proportional to the creatinine concentration in the reaction mixture.(Package insert: Creatinine plus ver.2. Roche Diagnostics; 07/2024)

 

Calcium:

Calcium ions react with 5-nitro-5'-methyl-BAPTA (NM-BAPTA) under alkaline conditions to form a complex. This complex reacts in the second step with EDTA. The change in absorbance is directly proportional to the calcium concentration and is measured photometrically.(Package insert: CA2 reagent. Roche Diagnostics; 08/2023)

 

Glucose:

Hexokinase catalyzes the phosphorylation of glucose to glucose-6-phosphate by ATP. Glucose-6-phosphate dehydrogenase oxidizes glucose-6-phosphate in the presence of nicotinamide adenine dinucleotide phosphate (NADP) to gluconate-6-phosphate. No other carbohydrate is oxidized. The rate of NADPH formation during the reaction is directly proportional to the glucose concentration and is measured photometrically.(Package insert: Glucose HK Gen.3. Roche Diagnostics; 07/2024)

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

84132

84295

82435

82374

84520

82565

82310

82947

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BMAMA Basic Metabolic Panel, S 24321-2

 

Result ID Test Result Name Result LOINC Value
AGAP Anion Gap 33037-3
BUN Bld Urea Nitrog(BUN), S 3094-0
CL Chloride, S 2075-0
GLURA Glucose, Random, S 2345-7
HCO3 Bicarbonate, S 1963-8
CA Calcium, Total, S 17861-6
NAS Sodium, S 2951-2
KS Potassium, S 2823-3
CRTSA Creatinine, S 2160-0
EGFR1 Estimated GFR (eGFR) 98979-8