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Test Code HKC Hexokinase Enzyme Activity, Blood


Specimen Required


Only available as part of a profile. For more information see:

-HAEV1 / Hemolytic Anemia Evaluation, Blood

-EEEV1 / Red Blood Cell (RBC) Enzyme Evaluation, Blood


Useful For

The evaluation of individuals with Coombs-negative chronic hemolysis

Method Name

Only available as part of a profile. For more information see:

-HAEV1 / Hemolytic Anemia Evaluation, Blood

-EEEV1 / Red Blood Cell (RBC) Enzyme Evaluation, Blood

 

Kinetic Spectrophotometry (KS)

Reporting Name

Hexokinase, B

Specimen Type

Whole Blood ACD-B

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD-B Refrigerated 20 days

Reject Due To

Gross hemolysis Reject

Clinical Information

Hexokinase (HK) is the first enzymatic step in glycolysis, converting glucose to glucose-6-phosphate. Hexokinase deficiency (OMIM 235700) is a rare cause of chronic nonspherocytic hemolytic anemia and its inheritance is autosomal recessive. Clinically significant HK deficiency manifests in early onset anemia with variable severity ranging from mild to severe. Some patients show neurologic impairment of which the mechanism is unclear.

Reference Values

Only available as part of a profile. For more information see:

-HAEV1 / Hemolytic Anemia Evaluation, Blood

-EEEV1 / Red Blood Cell (RBC) Enzyme Evaluation, Blood

 

≥12 months: 0.7-1.7 U/g Hb

Reference values have not been established for patients who are younger than 12 months.

Interpretation

Clinical correlation or genetic confirmation may be required to establish hexokinase (HK) deficiency as a cause of hemolytic anemia as the assayed activity level in confirmed cases can vary from markedly decreased to borderline normal levels due to a compensated increase in enzyme by reticulocytes. Comparison of HK activity levels to other red blood cell enzyme activity can be very useful. Heterozygous individuals have moderately decreased to low normal HK levels and are expected to be clinically unaffected. 

 

Increased HK activity may be seen when reticulocytes are increased and is not supportive of a diagnosis of HK deficiency.

Cautions

Recent transfusion may mask the patient's intrinsic enzyme activity and cause unreliable results.

 

Hexokinase (HK) activity level can vary from markedly decreased to borderline normal levels in affected individuals due to a compensated increase in enzyme by reticulocytes. Comparison of hexokinase activity levels to other red blood cell enzyme activity can be very useful.

Method Description

Hexokinase catalyzes the reaction of adenosine triphosphate (ATP) and glucose to glucose 6-phosphate (G6P) and adenosine diphosphate (ADP). In this assay the formation of G6P is measured by linking its further oxidation to 6-phosphogluconate to the reduction of nicotinamide adenine dinucleotide phosphate (NADP[+]) through the glucose-6-phosphate dehydrogenase reaction. The increase in absorbance, which occurs as NADP(+) is reduced to NADPH, is measured spectrophotometrically at 340 nm on an automated chemistry analyzer.(Beutler E: Red Cell Metabolism: A Manual of Biochemical Methods. 3rd ed. Grune and Stratton; 1984:38-40; van Solinge WW, van Wijk: Enzymes of the red blood cell. In: Rifai N, Horvath AR, Wittwer CT: eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:chap 30)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82657

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HKC Hexokinase, B 49216-5

 

Result ID Test Result Name Result LOINC Value
HKCL Hexokinase, B 49216-5

Day(s) Performed

Weekly

Report Available

5 days