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HelpTest Code LADV Adenovirus, Molecular Detection, PCR, Varies
Reporting Name
Adenovirus PCRUseful For
Aiding in the diagnosis of adenovirus infections
Method Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesNecessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Sources: Pleural, peritoneal, ascites, pericardial, or amniotic
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate
Container/Tube: Sterile container
Specimen Volume: 1 mL
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Stool
Supplies: Stool Collection Kit, Random (T635)
Container/Tube: Sterile container
Specimen Volume: 1 g
Specimen Type: Swab
Supplies: M4-RT (T605)
Sources: Nasal, throat, respiratory, genital, or ocular
Container/Tube: Multimicrobe media (M4-RT) and Eswabs
Specimen Volume: Entire specimen
Collection Instructions: Place swab back into a multimicrobe media (M4-RT, M4, or M5).
Specimen Type: Tissue
Supplies: M4-RT (T605)
Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, or M5)
Specimen Volume: Entire collection
Collection Instructions: Collect fresh tissue specimen.
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 1 mL
Collection Instructions: Collect a random urine specimen.
Specimen Minimum Volume
Body fluid and respiratory specimens: 0.5 mL
Spinal fluid and urine: 0.3 mL
Stool: 0.5 g
Swab or Tissue: See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reject Due To
| Calcium alginate-tipped swab Wood swab Transport swab containing gel |
Reject |
Reference Values
Negative
Day(s) Performed
Monday through Friday
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LADV | Adenovirus PCR | 39528-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC65 | Specimen Source | 31208-2 |
| 89074 | Adenovirus PCR | 39528-5 |
Clinical Information
Human adenoviruses cause a variety of diseases, including pneumonia, cystitis, conjunctivitis, diarrhea, hepatitis, myocarditis, and encephalitis. In humans, adenoviruses have been recovered from almost every organ system. Infections can occur at any time of the year and in all age groups. Currently, there are over 50 adenovirus serotypes that have been grouped into 6 separate subgenera.
Although adenovirus can be recovered in cell culture, it can take up to 3 weeks for the virus to be identified by culture methods (Mayo Clinic's shell vial culture provides more rapid results, reported at 2 and 5 days). Serological tests have faster turnaround times but can be less sensitive compared to culture. Polymerase chain reaction assays offer a rapid, specific, and sensitive means of diagnosis by detecting adenovirus DNA.
Interpretation
A positive result indicates the presence of adenovirus DNA in the clinical specimen.
A negative result does not rule out the presence of adenovirus because viral DNA may be present at levels below the detection limits of this assay.
Cautions
Test results should be used as an aid in diagnosis and should not be considered diagnostic in themselves.
Although the reference range is generally considered to be "Negative" for this assay, adenovirus DNA may be detected from asymptomatic individuals in certain settings. This assay should only be used to test patients with clinical history and symptoms consistent with adenovirus disease, and is not used to screen healthy patients.
Method Description
Respiratory, swabs, stools, tissues, plasma, and urine samples are processed according to specimen source. Viral nucleic acid is extracted by the MagNA Pure automated instrument (Roche Applied Science). Primers and fluorescence resonance energy transfer (FRET) probes target a relatively conserved 185 base-pair region of the adenovirus penton gene. The LightCycler instrument (Roche Applied Science) amplifies and monitors the development of target nucleic acid sequences after the annealing step during polymerase chain reaction (PCR) cycling. This automated PCR system rapidly detects amplicon development through stringent air-controlled temperature cycling in capillary cuvettes. The detection of amplified products is based on the FRET principle. For FRET product detection, a hybridization probe with a donor fluorophore, fluorescein, on the 3'-end is excited by an external light source and emits light that is absorbed by a second hybridization probe with an acceptor fluorophore, LC-Red 640, at the 5'-end. The acceptor fluorophore then emits a light of a different wavelength that can be measured with a signal that is proportional to the amount of specific PCR product.(Unpublished Mayo method)