Sign in →

Test Code TAPNS Tapentadol and Metabolite, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (Serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.0 mL Serum

Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.


Useful For

Monitoring medication compliance in patients who are prescribed tapentadol

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Tapentadol and Metabolite, S

Specimen Type

Serum Red

Specimen Minimum Volume

Serum: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 14 days
  Frozen  28 days
  Ambient  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Clinical Information

Tapentadol is a centrally acting opioid analgesic which is utilized in the treatment of moderate to moderately severe pain. This drug is rapidly absorbed and has dual mechanism of action, combining mu-opioid receptor agonism with noradrenaline reuptake inhibition in the same molecule. The primary metabolite of tapentadol is N-desmethyltapentadol. The test will be primarily used by physicians to determine if patients are compliant with the medication.

Reference Values

Tapentadol:

Therapeutic: 5-300 ng/mL

 

N-desmethyltapentadol:

No therapeutic range established

 

Cutoff concentrations by liquid chromatography tandem mass spectrometry:

Tapentadol: 0.5 ng/mL

N-desmethyltapentadol: 0.5 ng/mL

Interpretation

Serum concentrations for pain relief typically fall in the range of 5-300 ng/mL, but depend heavily on dose, frequency, and individual patient factors.

Cautions

Specimens collected in serum gel tubes are not acceptable because the drug can absorb onto the gel and lead to falsely decreased concentrations.

Method Description

Serum samples are mixed with internal standard and extracted by protein crash with methanol. The supernatant after centrifugation is diluted with Clinical Laboratory Reagent Water. The sample is then analyzed by liquid chromatography tandem mass spectrometer.(Unpublished Mayo method)

Day(s) Performed

Tuesday

Report Available

3 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TAPNS Tapentadol and Metabolite, S In Process

 

Result ID Test Result Name Result LOINC Value
624021 Tapentadol 59354-1
624022 N-desmethyltapentadol In Process