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HelpTest Code TRCNG Trichinella Antibody, IgG, Serum
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Useful For
As an adjunct in the diagnosis of trichinosis
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Trichinella Ab, IgG, SSpecimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 30 days | |
| Refrigerated | 5 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-inactivated | Reject |
Clinical Information
Trichinosis is an infection by the nematode parasite, Trichinella spiralis. The infection is acquired by ingestion of larvae in inadequately cooked, contaminated meat, especially pork, bear, and walrus meat. After ingestion, acid-pepsin digestion in the stomach liberates the larvae, which develop into adult worms in the small intestine. After fertilization, the female worm produces larvae that penetrate the mucosa and seed the skeletal muscles via the blood stream. The larvae coil and encyst in muscle fibers, remaining viable for up to several years.
Diarrhea is the most common symptom associated with intestinal infection with adult worms. Fever, periorbital swelling, muscle pain and swelling, pulmonary symptoms, and rash develop during systemic invasion by the larvae.
Reference Values
Negative
Reference values apply to all ages.
Interpretation
Positive:
Results are suggestive of current or past infection with Trichinella spiralis. Results should be used in conjunction with clinical, epidemiologic and other laboratory tests to diagnose current infection.
Borderline:
Recommend follow-up testing in 10 to 14 days if clinically indicated
Negative:
No antibodies to Trichinella spiralis detected. Repeat testing in 2 to 3 weeks if clinically indicated.
Cautions
Antibodies to Trichinella spiralis may not be detectable until 3 weeks after the onset of infection. Therefore, negative results in suspicious cases should be followed by repeat testing in several weeks.
Sensitivity of the test may also be affected by the level of invasive disease, with patients who have lower parasitic loads having a greater risk of being negative by the assay.
Cross reactions with antibodies against Toxocara canis are possible.
Method Description
Microtiter strip wells are precoated with Trichinella spiralis ES-antigens (excretory/secretory antigens) to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase-labelled protein A conjugate is added. This conjugate binds to the captured Trichinella spiralis-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of Trichinella spiralis specific antibodies in the specimen. Sulfuric acid is added to stop the reaction. This produces a yellow end point color. Absorbance alternative 450 nm is read using an enzyme-linked immunosorbent assay microwell plate reader.(Package insert: Trichinella spiralis ELISA IgG. Gold Standard Diagnostics; 11/02/2021)
Day(s) Performed
Thursday
Report Available
1 to 7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86784
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TRCNG | Trichinella Ab, IgG, S | 6563-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TRCNG | Trichinella Ab, IgG, S | 19253-4 |